RESUMO: A co-digestão anaeróbia dos resíduos é uma alternativa para aumentar a produção de metano (CH4) e melhorar a qualidade do biofertilizante. Este estudo teve como objetivo avaliar a melhor condição de coDA dos dejetos de suínos (DS), acrescidos de batata doce (BD) ou mandioca (M) em diferentes inclusões, em biodigestores semi-contínuos. Inicialmente, utilizando biodigestores batelada, foi realizado ensaio de coDA dos DS com BD ou M, adotando-se as relações C/N iguais a 10/1, 13/1, 17/1, e 22/1. Com base nos resultados elegeu-se a relação C/N de 10/1, como sendo a proporção que apresentou maiores reduções de sólidos voláteis (SV) e produções específicas de biogás. A partir destes resultados, efetivou-se o experimento em biodigestores semi-contínuos, composto por três tratamentos (controle (DS), BD 10/1 e M 10/1) com cinco repetições cada, e tempo de retenção hidráulica (TRH) de 30 dias. Foram mensuradas as reduções de sólidos totais (ST) e sólidos voláteis (SV), produções de biogás e CH4, alcalinidade e acidez volátil. O tratamento controle diferiu dos demais e apresentou menor desempenho no rendimento de biogás (752 LN.kgSVadic -1), CH4 (449 LN.kgSVadic -1) e concentração de CH4 (59,7%). Os tratamentos em coDA (BD e M) não diferiram entre si para o rendimento de biogás (901 e 883 LN.kgSVadic -1) e CH4 (590 e 547 LN.kgSVadic -1), no entanto diferiram na concentração de CH4 (65,5 e 61,9%, respectivamente). Os tratamentos apresentaram médias gerais de reduções de 76,1 e 85,9% para ST e SV, respectivamente, não sendo encontrada diferença estatística entre eles. A coDA do DS com BD ou M aumentou a produção e a qualidade do biogás, elevando a concentração de CH4 no mesmo.

ABSTRACT: Anaerobic co-digestion (AcoD) of waste is a method of increasing methane (CH4) yield and improving biofertilizer quality. This study aimed to evaluate the best AcoD conditions for swine manure (SM) with sweet potato (SP) or cassava (C) in different amounts in semi-continuous biodigesters. Initially, using batch biodigesters, an AcoD test of the SM with SP or C was performed, adopting carbon/nitrogen (C/N) ratios of 10/1, 13/1, 17/1, and 22/1. Based on the results, a C/N ratio of 10/1 was chosen, which was the proportion that resulted in the highest reduction of volatile solids (VS) and specific biogas production. From these results, the experiment was carried out in semi-continuous biodigesters, consisting of three treatments (control (SM), SP 10/1, and C 10/1) with five replicates and a hydraulic retention time (HRT) of 30 days. Total solid (TS) and volatile solid (VS) reductions, biogas and CH4 yields, alkalinity, and volatile acidity were measured. The control treatment differed from the others and resulted in decreased biogas yield (752 LN.kgVSadded -1), CH4 (449 LN.kgVSadded -1), and CH4 content (59.7%). The AcoD treatments (SP and C) did not differ significantly for biogas yield (respectively, 901 and 883 LN.kgVSadded -1) and CH4 (respectively, 590 and 547 LN.kgVSadded -1); however, they differed in CH4 content (65.5% and 61.9% respectively). The treatments showed general reduction averages of 76.1% and 85.9% for TS and VS, respectively, with no statistical difference found between them. The AcoD of the SM with SP or C increased the production and quality of the biogas, increasing the concentration of CH4 therein.

ABSTRACT: The composting of laying hen manure is an efficient way of treating waste and producing an organic fertilizer with excellent characteristics; however, the high N losses reduce its concentration in the resulting fertilizer. Associating this residue with crude glycerin (CG) promotes a better C:N ratio and contributes to higher N retention during composting, with improvements in solids reduction and production of organic fertilizer with higher concentration of this nutrient. We carried out this research considering the hypothesis that adding CG – a byproduct of biodiesel production – to laying hen waste composting provides better N retention in the fertilizer generated. Increasing CG doses (0, 3, 6, 9, and 12 % in relation to the fresh mass in the windrows) were added to the laying hen manure with windrows composted for 70 days. A reduction by 100 % in total and thermotolerant coliforms occurred at all levels of CG addition. The maximum reductions in total (TS) and volatile (VS) solids were 64 and 76 %, respectively, and occurred with the addition of 6 % CG. The windrows that did not receive CG had higher N losses during the process (32 %) while the highest N concentration in the compost was 5 % at 6 % CG. We identified that adding 6 % CG to the laying hen manure in co-composting increases the reductions in TS and VS and provides higher N retention, which improves the quality of the organic fertilizer.

RESUMO: Objetivou-se encontrar a melhor dose de inclusão de óleo residual durante a co-digestão de substratos preparados com dejetos de bovinos leiteiros e suínos, com o intuito de maximizar as reduções de sólidos e as produções de biogás. Foram realizadas no abastecimento e desabastecimento dos biodigestores as análises de sólidos totais (ST), sólidos voláteis (SV) e fibra em detergente neutro (FDN), enquanto as mensurações de biogás duas vezes por semana. A máxima redução de SV (51,4%) foi possibilitada pela inclusão de até 54,6g de óleo de descarte kg dejetos-1, já as máximas degradações de FDN ocorreram com adições de até 69,4g de óleo de descarte kg dejetos-1. As inclusões de até 64g de óleo de descarte kg dejetos-1 possibilitaram produções específicas de biogás de 291,4 e 251,0 L de biogás.kg SV-1 adicionado, para substratos contendo dejetos de suínos e bovinos respectivamente. A adição de óleo em doses entre 45,1 e 69,4g.kg-1 a substratos contendo dejetos de bovinos ou suínos maximiza as reduções dos constituintes sólidos e fibrosos bem como potencializa as produções específicas de biogás, sendo que o dejeto de suíno ainda suporta maiores doses de inclusão de óleo residual.

ABSTRACT: This study aimed to obtain the best dose of waste cooking oil inclusion for the co-digestion of substrates prepared with dairy cattle and swine manure in order to maximize solids reductions and biogas yield. Analyses of total solids (TS), volatile solids (VS), and neutral detergent fiber (NDF) were performed during the loading and unloading of digesters, while biogas yield was measured twice a week. The maximum reduction in VS (51.4%) was reached with the inclusion of up to 54.6g waste cooking oil.kg manure-1. Maximum NDF degradation occurred with inclusions of up to 69.4g waste cooking oil.kg manure-1. Inclusions of up to 64g of waste cooking oil.kg manure-1 provided specific biogas yield of 291.4 and 251.0L biogas.kg VS-1 added to substrates with swine and cattle manure, respectively. Adding oil at doses between 45.1 and 69.4g waste cooking oil kg manure-1 to substrates composed of cattle or swine manure maximizes reductions of solids and fibrous constituents and enhances specific biogas yield. Furthermore, swine manure supports higher doses of waste cooking oil.

ABSTRACT Anaerobic digestion of crude glycerin (CG) along with animal waste has been an excellent option for increasing the production of biogas and methane to achieve efficiency in the treatment of both residues. This study aimed to evaluate improvements in specific productions of biogas and methane, reductions in solid and fibrous components in substrates prepared with dairy cattle manure and CG (containing 14 % glycerol). With these residues, experimental substrates were prepared and placed in 25 batch digesters. Initial content of the TS in the influent was 4 % and CG was added in increasing doses (0, 5, 10, 15 and 20 % relative to total solids (TS) of the influent). Results were submitted to ANOVA and orthogonal contrasts to assess the effects of linear and quadratic order and thereby estimate the optimal CG doses through the adjusted models. The highest values for specific production of methane (0.19 and 0.26 L g−1 of TS and volatile solids (VS) added, respectively) were reached with the CG inclusions of 6 and 8 %, respectively. Total production of biogas with the inclusion of 6 % CG was 11 % higher when compared to the control treatment. The largest reduction in VS (48 %) was achieved with the addition of 4 % CG. Addition of CG at levels between 3 and 8 % improved the efficiency of the process of anaerobic digestion with dairy cattle manure.

ABSTRACT The effect of additives used in the feed of broilers on anaerobic bio-digestion of poultry litter was evaluated. Four diets were used: NC: negative control; DFM: NC + 500 ppm direct-fed microbials (DFM) containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm xylanase); DFM+E: ENZ + DFM. Substrates for the anaerobic bio-digestion were prepared with litter from each treatment, containing 4 % total solids (TS). These were used in 16 continuous bio-digesters with a 2 kg d−1 load, to determine the production and potential biogas production and composition during an 85-day period. Influent and effluent samples were collected for the amounts of TS and volatile solids (VS), fiber fraction (neutral detergent fiber [NDF], acid detergent fiber [ADF] and lignin), nutrients (N, P and K), and total and thermotolerant coliforms to be determined. For all treatments a reduction in the following effluents was observed as follows: TS (49, 48, 48 and 50 %) VS (70, 54, 55 and 62 %) NDF (91, 90, 95 and 96 %) ADF (89, 88, 93 and 94 %) and lignin (80, 76, 89 and 88 %). The efficiency of the treatment for coliforms in bio-digesters was higher than 90 % in the 85-day period in all treatment groups. There was a reduction in biogas and methane production when DFM (5500 and 4000 mL) and DFM + E (5800 and 4100 mL) were used, compared to treatments NC (6300 mL and 4400) and ENZ (6400 and 4500 mL). The potential production of reduced TS and VS was higher in ENZ (1:00 and 1.74 106 mL kg−1) when compared to NC (0.88 and 1:02 106 mL kg−1), DFM (0.80 and 1:40 106 mL kg−1) and DFM + E (0.88 1:25 and 106 mL kg−1). The additives did not affect the percentage of methane production, and all treatments showed values higher than 70 %. Adding enzymes to the diet of broilers influences the litter characteristics and, as a consequence, increases biogas production. The addition of DFM and DFM + E to broiler diets reduced biogas and methane production.

Em virtude da grande demanda por proteína de origem animal, tem-se aumentado a produção de frangos de corte e consequentemente a geração de resíduos provenientes do abate de aves, sendo necessário o desenvolvimento de técnicas que permitam o aproveitamento e reciclagem desses materiais. Objetivou-se com a execução deste trabalho avaliar a eficiência da compostagem no tratamento e reciclagem do resíduo sólido de abatedouro avícola. Utilizou-se resíduo sólido de abatedouro avícola comercial composto por fragmentos de vísceras, tecido muscular, adiposo e ósseo, sangue coagulado e penas e, como fonte de carbono, a casca de arroz. Montou-se uma leira com 1,5m3 de volume inicial, na qual foram monitorados os parâmetros: temperatura, teores de sólidos totais (ST), voláteis (SV), N, P, K, carbono orgânico (C), matéria orgânica compostável (MOC), matéria orgânica resistente à compostagem (MORC), demanda química de oxigênio (DQO), massa e volume enleirados, número mais provável (NMP) de coliformes totais e termotolerantes, bem como suas reduções durante o processo. A temperatura máxima atingida no centro da leira foi de 53,3ºC (média semanal), já as reduções de massa de ST e SV e volume durante o processo de pré-compostagem foram de 36,1; 44,3 e 23,3%, respectivamente, e, durante o processo de compostagem, foram de 21,8; 23,8 e 4,4%. A baixa redução do volume das leiras pode estar associada à alta concentração de MORC (40,1%) que pode ser principalmente relacionada à qualidade da fonte de carbono. O processo promoveu satisfatórias reduções totais de ST, SV e volume, sendo, respectivamente, 50,1; 57,5 e 26,7%. No entanto, foram observadas reduções de 43% na quantidade de nitrogênio presente no composto final. Apesar das reduções de nitrogênio, a compostagem demonstrou ser um método eficiente no tratamento dos resíduos sólidos de abatedouro avícola.

The great demand for animal protein was responsible for the increase on the broilers production and hence, the generation of waste from the poultry slaughter was increased as well, which in turn, propelled the development of techniques that allow the reuse and recycling of these wastes. The objective of this study was to evaluate the efficiency of composting on the treatment and recycling of solid waste from poultry slaughterhouse. The solid waste was from a commercial poultry slaughterhouse and was composed of viscera, muscle, fat, bone, blood and feathers that was mixed with a source of carbon, rice husk. Initially, a windrow with a volume of 1.5m3 was built, and then some parameters were monitored: temperature, total solids (TS), volatile (VS), N, P, K, organic carbon (C), composting organic matter (COC), organic matter resistant to composting (MORC), chemical oxygen demand (COD), mass and volume of the windrow, most probable number (MPN) of total and fecal coliforms, as well as their reductions during the process. The maximum temperature reached in the center of the windrow was 53.3°C (weekly average) since reductions of weight of TS and VS and volume during the pre-composting were 36.1, 44.3 and 23.3%, respectively and during the composting process was 21.8, 23.8 and 4.4%. The low volume reduction can be associated with high concentrations of MORC (40.1%) which can be mainly related to the quality of the carbon source. The process produced satisfactory total reductions of TS, VS and volume that were respectively, 50.1, 57.5 and 26.7%. However reductions were observed in 43% of amount of nitrogen in the final compound. Despite reductions in nitrogen content, composting proved to be an effective method in the treatment of solid waste from poultry slaughterhouse.

O objetivo do trabalho foi verificar a eficiência da co-digestão anaeróbia dos dejetos suínos com crescentes níveis de adição de óleo de descarte e lipase. Foram testadas quatro doses de óleos (8, 12, 16 e 20g kg-1 de substrato) e 3 doses de lipase (1, 2 e 3g kg-1 de substrato). Os parâmetros avaliados foram: redução de sólidos totais (ST), sólidos voláteis (SV) e número mais provável (NMP) de coliformes totais (CT) e termotolerantes (CTT), além do pH, demanda química de oxigênio (DQO), produção e potenciais de produção de biogás. Os resultados demonstraram que houve influência das adições de óleo e lipase sobre as reduções de ST SV e pH do efluente, seguindo um modelo de predição linear e decrescente. As reduções do NMP de CT e CTT foram mais elevadas nos tratamentos com maior adição de óleo e lipase. A produção de biogás apresentou uma correlação negativa com as adições de lipase, sendo que estas proporcionaram efeito linear decrescente sobre os potenciais de produção de biogás/kg de dejeto, ST e SV adicionado. As adições de óleo e lipase não proporcionam efeitos favoráveis sobre as produções de biogás.

The goal this paper was to verify the efficiency of the anaerobic co-digestion of manure with increasing levels of added oil disposal and lipase. Were tested four levels oil (8, 12, 16 and 20 g kg-1 substrate) and 3 doses of lipase (1, 2, 3g-1 kg of substrate). The parameters evaluated were: reduction of total solids (TS), volatile solids (VS) and most probable number (MPN) of total coliforms (TC) and thermophilic (TTC), in addition to pH, chemical oxygen demand (COD), production and potential biogas production. The results demonstrated the influence of additions of oil and lipase to the reductions in SV and ST effluent pH, following a model of linear prediction and descending. The reductions in MPN of TC and TTC were higher in treatments with more adding oil and lipase. Biogas production showed a negative correlation with the additions of lipase, and these yielded linear effect on the potential of biogas/kg of manure, ST and SV added. The additions of oil and lipase did not provide favorable effects on biogas production.

A adubação orgânica pode ser uma alternativa viável na produção de gramíneas forrageiras, no entanto poucas são as informações referentes às doses e à composição dos principais adubos orgânicos. O objetivo do trabalho foi verificar a influência das diferentes doses de composto orgânico produzido com dois tipos de cama de frango sobre as características produtivas, morfogênicas e estruturais de Brachiaria brizantha, cv 'Piatã'. O experimento foi conduzido em casa de vegetação em delineamento inteiramente casualizado, em esquema fatorial com parcela subdividida no tempo. As parcelas foram compostas por seis tratamentos: dois tipos compostos (cama de frango a base de cana de açúcar ou napier) em três doses (100, 200 e 300kg ha-1 equivalente N) e as subparcelas pelos quatro períodos de cortes. Os compostos foram aplicados em dose única, após o corte de uniformização, nas quantidades de: 11,36 e 11,83, 22,73 e 23,67, 34,09 e 35,50g vaso-1 para os compostos de cama de frangos a base de cana de açúcar e capim napier, respectivamente, que equivalem às doses de 100, 200 e 300kg ha-1 de N. As variáveis mensuradas foram: produção de matéria seca (PMS), taxa de aparecimento de folha (TApF), taxa de alongamento de folha (TAlF), filocrono, taxa de alongamento de pseudocolmo (TAlC), comprimento final de folha (CFF) e números de folhas verdes (NFV). Não foi observada diferença significativa entre os tipos de composto e na interação composto x dose. Dessa forma, ambos poderiam ser utilizados sem que ocorresse prejuízo no aproveitamento dos nutrientes pelas plantas avaliadas no experimento. Houve diferença significativa entre a PMS, TApF, filocrono, TAlF e TAlC, NFV e TFF em função das doses crescentes de nitrogênio, segundo um modelo linear de predição. Também foi observado efeito dos períodos de corte, em que os cortes realizados no verão apresentaram melhor desempenho sobre as características morfogênicas e estruturais.

The organic fertilizer can be a viable alternative in the production of forage grasses, however there is little information regarding doses and composition of the major organic fertilizers. The aim of this paper was to verify the influence of the different doses of organic compost produced from two types of poultry litter on the structural, morphogenetic and productive characteristics of Brachiaria brizantha cv 'Piatã'. The experiment was conducted in a greenhouse in a completely randomized design in a factorial scheme l with parcel subdivided in time. The parcels were composed by six treatments: two types of compost (poultry litter based in sugar cane and napier grass) in three doses (100, 200 and 300kg ha-1 equivalent N.) and the subparcels by the four different periods of cut. The composts were applied in a unique dose, after the uniformization cut, at the quantities: 11,36 and 11,83, 22,73 and 23,67, 34,09 and 35,50 g pot-1 for the poultry litter based in sugar cane and napier grass, respectively which are equivalent to the rates of 0, 100, 200 and 300kg ha-1 of N. The variables measured were: dry matter production (DMP), leaf appearance rate (LApR), phyllochron, leaf elongation dose (LER) and shoots elongation rate (SER), number of green leaves (NGL), final size of the leaf (FSL). No significant difference between the types of composts and in the interaction compost x doses was observed, thus, both could be used without the risk of loss in the use of the nutrients by the plants evaluated in the experiment. There was a significant difference between the DMP, LApR, phyllochron, LER, SER, NGL and FSL because of the increasing rates of nitrogen, followed by a linear model of prediction. The effect of the periods of slaughter was also observed, where the slaughters carried out in the summer presented a better performance over the morphogenetic and structural features evaluated.

O objetivo do trabalho foi avaliar diferentes doses de efluente de abatedouro avícola para proporcionar melhorias nas características produtivas, morfogênicas e estruturais do capim-piatã. O experimento foi conduzido em casa de vegetação, adotando-se delineamento inteiramente casualizado, em que foram testadas cinco doses de efluentes: 324, 648, 972, 1.296 e 1620m³ ha-1 ou equivalente a 50, 100, 150, 200 e 250kg ha-1 de N. As variáveis mensuradas foram: produção de matéria seca (MS), taxa de aparecimento de folha (TApF), taxa de alongamento de folha (TAlF), filocrono, taxa de alongamento de pseudocolmo (TAlC), comprimento final de folha (CFF) e números de folhas verdes (NFV). A produção de MS seguiu um modelo linear de predição em função das doses efluente avícola, em que o tratamento com 250kg ha-1 de N foi 55% maior, quando comparado com o tratamento de 50kg ha-1 de N. Todas as características morfogênicas e estruturais avaliadas com exceção do filocrono apresentaram comportamento linear positivo. Dessa forma, o efluente de abatedouro avícola pode ser utilizado como uma alternativa para adubação do capim-piatã, pois este respondeu de maneira crescente até a dose máxima testada.

The organic fertilizer is considered a viable alternative for the production of forage grasses. However there is little information about the rates and composition of the organic fertilizers. According to this assumption the objective of this study was to figure out the best dose of effluent from poultry processing plants in order to improve the structural, morphogenetic and productive characteristics of Piata palidadegrass. The experiment was carried out at a greenhouse and performed in a completely randomized design in which five doses of effluent, 324, 648, 972, 1296 and 1620m³ ha-1 were tested and equivalent to 50, 100, 150, 200 and 250kg ha-1 de N respectively. The measured variables were: dry matter (DM), leaf appearance rate, leaf elongation rate, phyllochron, pseudo stem elongation rate, final leaf length and numbers of green leaves. The production of DM presented a linear prediction model according to the N rates, while the treatment with 250kg ha-1 de N was 55% higher when compared to the treatment with 50kg ha-1 de N. All morphogenetic and structural characteristics that were evaluated presented a positive linear fashion, except the phyllochron. Thus, the effluent from poultry processing plant may be used as an alternative to fertilization of the 'Piata' palisadegrass because this responded by increasing the maximum dose tested.

The objective of this study was to evaluate the efficiency of anaerobic digestion on the treatment of effluent from poultry slaughterhouse. The experiment was conducted at the Laboratory of Waste Recycling from Animal Production/FCA/UFGD. During four weeks, eight experimental digesters, semi-continuous models, were loaded and set according to the hydraulic retention time (HRT) of 7, 14, 21 and 28 days, and according to the solid fraction treatment, separated with 1 mm sieve or without separation. The average weekly production of biogas and methane as well as the methane concentrations, the potential production per amount of chemical oxygen demand (COD) added and reduced, the concentrations of N, P and K at the beginning and end of process, and the most likely numbers of total and thermotolerant coliforms were evaluated. For data analysis, a completely randomized design was performed in a 4 × 2 factorial arrangement (4 HRT: 7, 14, 21 and 28 days and separation with 1 mm sieve or without separation), with repetition over time. The highest production of biogas and methane was statistically significant for the HRT of 7 and 14 days (5.29 and 2.38 L of biogas and 4.28 and 1.73 L of methane, respectively). There was an interaction between HRT and the separation of the solid with sieve and the highest production was obtained in the treatment without separation. Similar behavior was observed for the potential production with a maximum of 0.41 m³ methane.kg-1 COD added with an HRT of 7 days without separation of the solid fraction. The separation of the solid fraction is not recommended in the pretreatment of liquid effluent from poultry slaughterhouse, once the potential for production and production of methane and biogas were reduced with this treatment.

The objective of this study was to investigate the influence of different levels of biofertilizers from cattle and swine manure on the structural, morphogenetic and productive characteristics of Brachiaria brizantha cv. Piatã. The experiment was arranged in a completely randomized factorial design with split plots. The plots were defined by eight treatments: two biofertilizers (cattle and swine), four levels (0, 100, 200 and 300 kg N.ha-1) and subplots by four different cutting periods. The cutting for plant uniformity was performed at 45 days after sowing at 15 cm above the soil surface. The biofertilizeres were applied in a single level, after the cutting of plants, in rates of 0, 0.23 and 0.19, 0.45 and 0.38, 0.68 and 0.57 liters pot-1 for the biofertilizers from cattle and swine manure, respectively. These rates were also equivalent to levels of 0, 100, 200 and 300 kg N.ha-1. There was no significant difference between the types of biofertilizers as there was no interaction between them and the different levels, hence both biofertilizers could be applied without any loss of nutrient intake by the plants used in this experiment. There was a significant difference between the production of green and dry matter, the leaf appearance rate, phyllochron, leaf and pseudostem elongation rates, number of green leaves, final leaf length, number and weight of tillers, according to the increase of nitrogen rates, following linear prediction model. Effect of the cutting periods was also observed, once the plants harvested during the summer presented greater performance of structural and morphogenetic characteristics.